Effects of Tougu Xiaotong Capsules on the expression of Rac1 and Cdc42 in chondrocytes

doi:10.3969/j.issn.2095-4344.2014.42.005

Abstract

Abstract:

BACKGROUND: Tougu Xiaotong Capsule has pretty good clinical therapeutic effect on osteoarthritis of early and middle periods. However, the mechanism of Tougu Xiaotong Capsule is not fully clarified. The RhoA GTPases can regulate chondrocyte apoptosis and hypertrophy.
OBJECTIVE: To observe the Tougu Xiaotong Capsule on the expression of Rac1and Cdc42 in tumor necrosis factor-α-induced in vitro cultured rat articular chondrocytes, and to explore its mechanism of action for combating osteoarthritis.
METHODS: Knee cartilage of the 4-week-old Sprague-Dawley rats was used to stably establish in vitro culture system of chondrocytes. Passage 3 chondrocytes were identified by toluidine blue staining. Chondrocyte apoptosis was successfully induced by 20 μg/L tumor necrosis factor-α and then Tougu Xiaotong Capsule at different dosage (500, 100, 20 mg/L) was given after 24-hour incubation. MTT assay was used to detect cell survival, flow cytrometry to measure mitochondrial membrane potential, and western blot assay to determine the protein expression of Rac1, Cdc42, Bax and Bcl-2.
RESULTS AND CONCLUSION: Tougu Xiaotong Capsule could reduce tumor necrosis factor-α-induced apoptosis of chondrocytes to improve the survival rate of the cells, and at the same time, could down-regulate the protein expression of Rac1, Cdc42 and Bax and increase the protein expression of Bcl-2 significantly (P < 0.05). Tougu Xiaotong Capsule possibly plays a therapeutic efficacy on osteoarthritis by reducing promote apoptosis Rac1, Cdc42 and Bax expression and increasing apoptosis inhibiting gene Bcl-2 expression, thereby to inhibit apoptosis of chondrocytes.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: chondrocytes, apoptosis, drugs, Chinese HERBAL, rac1 GTP-binding protein, cdc42 GTP-binding protein

CLC Number:

R318

Ye Jin-xia, Wu Guang-wen, Li Xi-hai, Zheng Chun-song, Xu Hui-feng, Ye Hong-zhi, Liu Xian-xiang. Effects of Tougu Xiaotong Capsules on the expression of Rac1 and Cdc42 in chondrocytes[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(42): 6747-6751.

Figures/Tables 3

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